Flow cytometers, cell sorters and cell preparation systems. Find a flow cytometer for sale and auction at labx. This instrument is in great condition and has been verified by our company technical staff to. Amnis cellstream amnis flowsight amnis imagestream x mk ii guava easycyte guava muse.
For more detailed instructions on system operation details, please consult the millipore guava operation manual. Since the initial commercialization of flow cytometry fc and fluorescence activated cell sorting facs in 1968, they have undergone significant improvements. A practical guide for using flow cytometry and cell sorting, including extensive discussion on hardware, suppliers, reagents, and software. The guava easycyte flow cytometry systems are uncomplicated instruments that deliver complex cell analysis right on your benchtop. Guava easycyte mini from guava technologies biocompare. Millipore guava easycyte flow cytometer is available from the lab world group. The culmination of over a decade of flow cytometry expertise, these instruments use smaller sample, generate less waste, and are easier to use and maintain than traditional flow cytometers all while providing. For best performance, emd millipore corporation recommends that you use the medium flow rate, except for the guava cell cycle software module, which uses low or very low flow rates only. Find msds or sds, a coa, data sheets and more information. This technology offers some advantages for general flow cytometry but the instruments are different from traditional flow cytometry and the performance of legendplex is also different.
Setup procedure for guava easycyte with a single laser important note. Navios flow cytometer the navios offers up to three high powered solid state, independentlyfocused diode lasers with an innovative integrated optics flow cell design. Rapid, effective and userfriendly immunophenotyping of. Our group has used the guava extensively for well over a year. Guava easycyte ht bg flow cytometer shared equipment. This budgetfriendly instrument is capable of detecting up to 17 parameters with enhanced sensitivity and resolution. The guava easycyte plus guava technologies, hayward, ca, us is the first, personal, benchtop flow cytometer designed to address these limitations. Cytometry accuri c6 vs guava cytometersfeedback needed. With amnis chargecoupled device, timedelayed integration ccdtdi technology, the cellstream provides unparalleled high sensitivity in a highly customizable flow cytometer. Shared equipment authority sea we provide sophisticated research equipment and facilities to all members of the rice university community, as well as support and open instrumentation usage to outside companies, nanobioenviro startups and other universities upon availability. All guava instruments use microsyringebased fluidics, which eliminates the need for sheath fluid and enables the user to obtain accurate cell counts in the samples. The guava easycyte 5 flow cytometer offers absolute cell counts and five parameters 3 fluorescent colors, 2 light scatter, from an industry standard blue laser.
Some of our popular manufacturers include beckmancoulter, bd biosciences bectondickinson and guava. The navios flow cytometer offers a solution for advanced cytometry applications with workflows for high. Emd millipore has thoroughly tested the operation of guavasoft software on. Used guava technologies pca flow cytometer for sale. Guava incyte brings a new level of analytical power to flow cytometry. Setup procedure for guava easycyte with two lasers important. Plus, since the flow cell is selfaligning and user replaceable. Ion sphere particles quality assessment for the ion proton and ion s5 systems using the guava easycyte 5 benchtop flow cytometer publication number man0007496 revision b. If you created a worklist already, click start worklist. Blue 488 nm 150 mw and green 532 nm 100mw with 8 fluorescent detection channels, forward and side scatter.
It also enables other very powerful cell analysis and qc. We present the utter option of this ebook in pdf, txt, djvu, epub, doc forms. The culmination of over a decade of flow cytometry expertise, these instruments consume less sample, generate less waste and are easier to use and maintain than traditional flow cytometers all. The guava viacount, guava check, and guava easycheck software modules allow samples to be run only at the medium flow rate.
A guava easycyte ht 2laser flow cytometer millipore was used with excitation by the blue 488nm laser and the gain controls at their default settings yellow and green fluorescence or set to 9. You can change these default preference sets in the cytometer specific preferences. Attune acoustic focusing cytometer user guide 7 safety information or instrument safety information. The instrument also conforms to the ul and cancsa general requirements 61010. The instruments compact design is suited for basic research as well as advanced immune monitoring. Flow cytometers, reagents, software beckman coulter. Its superior sensitivity and resolution throughout all configurations give it the edge over other cytometry systems four times its size. Has anyone experience with the easycyte 5 flow cytometer from millipore.
Assay results with statistics automatically calculated and. Manual adjustments can also be made after data load by following these instructions. Millipore guava ht with plate handler protocol for multiplex. Guava easycyte instrument features easycyte flow cytometers use novel microcapillary technology that enables a compact userfriendly format, which is powerful enough to run your most complex assays. The first place to look for new and used flow cytometers. Navios is equipped with electronics that provide accurate and efficient digital signal processing at high event rates over a wide dynamic range of fluorescence intensities. All these features make guava easycyte mini a fully functional and very attractive flow cytometer. The flowsight and imagestream imaging flow cytometers add quantitative, microscopy imaging of every cell to your flow cytometry. Setup procedure for guava easycyte with a single laser.
Microcapillary flow cytometry at the heart of every guava system is a patented, microcapillary flow cell that eliminates the need for sheath fluid. Oct 07, 2016 millipore guava easycyte flow cytometer is available from the lab world group. Determinations of algal numbers using the guava flow cytometer. These simple fluidics enable a selfaligning flow cell that can be removed for cleaning by the user. Novocyte is a high performance benchtop flow cytometer designed for all levels of users and all types of laboratories. Phd graduate student kyoto prefectural university of medicine kyoto, japan. The guava easycyte ht doesnt require sheathing fluid, so you can. Plus, the flow cell is selfaligning and user replaceable to reduce downtime and service visits. Orflo technologies develops manufactures the most accurate and precise cell counters, cell qc analyzers, cell analyzer and flow cytometers. Ion sphere particles quality assessment user bulletin 7. When you choose a beckman coulter solution you receive the a high. The guava easycyte flow cytometry systems are easy to use and deliver complete and comprehensive cell analysisright on your benchtop.
The cytoflex flow cytometer, the first introduction to the cytoflex platform, provides the performance you need in an easy to use system allowing you to focus on the science, not the instrumentation. Flow cytometers life science classifieds new and used. The aim of this study was to adapt the immunohistochemical. The culmination of over a decade of flow cytometry expertise, these instruments consume less sample, generate less. Thermo fisher scientific certifies that the attune nxt flow cytometer conforms to relevant directives to bear the ce mark. The results we obtained are highly reproducible with superb intraexperiment variation coefficients. Emd millipore has thoroughly tested the operation of guavasoft software on windows xp, but. We are considering either accuri c6 or guava flow cytometers and we would greatly appreciate if we get a good feedback from those who used these machines for issues like running costs, maintanance and drawbacks of each. The navios ex flow cytometer offers a solution for advanced cytometry applications with workflows for high throughput laboratories. Guava easycyte flexible, intuitive, and affordable cytometry for all your needs watch an overview video on the guava easycyte systems our microcapillary flow cytometry systems are simpler to operate than traditional sheathfluid based instruments and are far easier to maintain.
Safety on page 68 for the complete the chemical safety alert words four safety alert words appear in life technolo gies user documentation at points in the document where you need to be aware of relevant hazards. Use the following metrics together to evaluate a sample, but not as. Millipore guava easycyte ht flow cytometer system available from the lab world group. Because the scale of flow data is relative, this is acceptable. Additional recommendations when collecting and analyzing your samples. The attune nxt flow cytometer is a class i laser product per center for devices and radiological health cdrh regulations and eniec 60825. However, many flow cytometers are expensive to purchase andor use, and many do not offer effective automated handling of samples, so that extensive operator time is. Luminex flow cytometers and cellular analysis instruments give you instant access to all facets of cellular phenotypes and morphology. Figure 11 bd facscalibur system with bd facs loader option. Attune nxt acoustic focusing cytometer the invitrogen attune nxt acoustic focusing cytometer is a benchtop cytometer that uses acoustic pressure to confine the injected particles to a tight central line as the sample passes through the optical cell for interrogation. In addition, it is equipped with a highthroughput sampler able to automatically measure samples directly from a 96well plate or via micro. Guava easycyte system users guide cytotoxicity apoptosis. Has anyone experience with millipores guava flow cytometer. The guava suite software package also includes easycheck for convenient qc of the instrument and a cleaning function to automate cleaning routines.
Comparison and evaluation of seven different bench. We also enjoyed its portability, since we were able to share it with other research groups and use it directly in the cell culture. To avoid exposure to laser radiation or electric shock, follow these guidelines. The guava instruments are based on microcapillary flow. The manual loader holds two separate tubes, allowing the user to progress.
May 31, 2017 millipore guava easycyte ht flow cytometer system available from the lab world group. The guava easycyte flow cytometers use up to three lasers 488, 642, and 405 nm, to achieve. Optimize your flow cytometry best practices for sample preparation, staining and analysis duration. Flow cytometer guava easycyte available for sale at the lab world group. The customizable laser and optical configurations of novocyte offer a high degree of flexibility while providing complex cell analysis capabilities. Emd millipore has thoroughly tested the operation of guavasoft software on microsoft windows xp and.
Ion sphere particles quality assessment for the ion proton. Guava suite software provides an intuitive user interface and offers acquisition. Spectrometers shared equipment authority rice university. We offer specialized instruments for routine or high complexity flow cytometric analysis designed with a deep understanding of the clinical workflow and the needs of the hematopathologist. The millipore guava easycyte ht flow cytometer is a flexible, compact and reliable high throughput benchtop system. The university of sydneys major institute for medical research seeks to answer questions about the human body in health and in illness. Flow cytometry cambridge scientific used lab equipment. Do not open the instrument or attempt to perform any internal maintenance. Guava easycyte ht bg system is a flow cytometer for performing flow cytometry experiments.
We offer an assortment of used and refurbished flow cytometers and cell sorters. Turn off the power to the system before removing the flow cell. Biosurplus has these instruments used for flow cytometry for sale with a wide range of features, a few of which are discussed here. Widespread use of flow cytometry for immunophenotyping in clinical veterinary medicine is limited by cost and requirement for considerable laboratory space, staff time, and expertise. As part of our complete flow cytometry solution, or service agreements are. It facilitates fully automated data acquisition in a 24tube or 96well plate format. Millipore, muse, and guava are registered trademarks or trademarks of. An optional, auto mated sample loader is also available. This translates into less complexity, smaller samples, and minimal waste, saving you both time and money. This flow cytometer system produces dependable data for a wide range of multicolor cellular and bead assays. Millipore guava easycyte ht flow cytometer system youtube. This flow cytometer system produces dependable data for a wide range of multicolor cellular and bead assays in a highly user friendly fashion and can handle 96well plates and up to 10 tubes in a single load. This is the next generation leveraging the laser and optics technology of our successful cytoflex research platform.
The guava easycyte flow cytometry systems are easy to use and deliver. The focus of this buyers guide is to discuss some of the key components and features of the instruments and to highlight some tips and considerations for selecting a flow cytometer system for your. The instrument accommodates 96well plates and up to 10 tubes in a single load. Rapid, precise, and accurate counts of symbiodinium cells using the guava flow cytometer, and a. Sorting has the advantage of isolating particular cells for further study away from the nonlabeled of cells.
Follow the software instructions if prompted to put required tubessolutions for instrument tray check. May 27, 2016 scientists have many instrumentation and reagent options when selecting andor purchasing a flow cytometer system to address their research needs. The guava muse flow cytometer is easy to use, fast and reliable, far less expensive than other bench top sytems. Guava easycyte flow cytometers from luminex corporation. The macsquant analyzer 16 the macsquant analyzer 16 is engineered to expand the revolutionary automation of the macsquant analyzer line of flow cytometers. Orflo uniquely integrates flow cytometry fluoursence detection with the coulter counter principle. Setup procedure for guava easycyte with two lasers. The guava easycyte ht system users guide provides detailed. Used flow cytometry cell counting sorting biosurplus. Guava incyte software includes features that can be applied to control user access to data files and methods, which is useful in multi user environments. Rapid, precise, and accurate counts of symbiodinium cells. Multiply the m1 cellsml by the original sample volume to calculate the total number of recovered isps.
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